FastQCFastQC Report
Fri 27 May 2022
EGAF00004977367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347092426
Sequences flagged as poor quality0
Sequence length74
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACA18613920.5362813650102524No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTA16979860.48920283843934986No Hit
CTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACA16118080.4643742932033902No Hit
GTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAAT15746620.4536722446372253No Hit
GCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTT14710540.4238219822174973No Hit
GCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCT13758240.39638548609527996No Hit
AGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCT12212320.35184634077840693No Hit
AAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAG11533970.33230255505488904No Hit
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGA11379760.32785964623728203No Hit
GCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGC10645290.306699000110132No Hit
GCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGC10626650.30616196736024426No Hit
GTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCC9932700.2861687336271636No Hit
GTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAG9917860.28574118180268215No Hit
GGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGC8857780.25519946090670387No Hit
GCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8783720.25306573529207466No Hit
CTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGT8744560.2519375055449928No Hit
GGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGA8219990.23682424000804905No Hit
CCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGT8049840.23192208751913243No Hit
AGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGA8047770.23186244922555585No Hit
GGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCCACC7718340.22237131731592438No Hit
GGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGA7643290.22020906903914983No Hit
AGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATA7334560.21131432006528428No Hit
GCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAG7227570.20823185579969988No Hit
GCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGA6996610.20157772039658392No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTG6959510.20050884083538026No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGG6880250.19822529921756346No Hit
GTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGC6802760.1959927526623701No Hit
GACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGT6641320.1913415419787927No Hit
GGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACC6358410.18319068708229316No Hit
CACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCC6306610.18169828920438616No Hit
CAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCC6288800.1811851693934687No Hit
ACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAA6197810.17856367744538454No Hit
AAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTAC6111320.1760718339615973No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACA6008450.1731080700677692No Hit
GCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC6003030.172951915695216No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACC5970720.17202103972156396No Hit
GGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGG5954300.17154796688072935No Hit
GCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAG5940380.17114692096450412No Hit
CTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAA5822810.1677596387539727No Hit
AGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCA5783620.16663054468379554No Hit
GCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACT5721160.16483102399935398No Hit
GACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGAT5626460.16210264409514946No Hit
ATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCC5621960.16197299563085252No Hit
GGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAG5564510.16031781690332822No Hit
GCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAAC5426940.1563543192959215No Hit
GTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCA5389730.155282270550035No Hit
GAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACG5353530.15423932068169072No Hit
GAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCT5346130.15402612098484686No Hit
GTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCCACCC5263570.1516475038265456No Hit
GGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGG5141440.1481288445055266No Hit
CTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC5103240.1470282730974948No Hit
ACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA5089530.14663327744293678No Hit
GTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACC5076400.14625499203488815No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCC5074280.14619391320281935No Hit
CAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCCACCCCAAGG4959830.14289652059420047No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACC4953770.14272192732894723No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA4903550.14127505046739336No Hit
CAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTA4844480.1395731982927222No Hit
GTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAG4810170.13858470077938262No Hit
CACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTC4767400.13735246415316477No Hit
CCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTT4714540.13582952685922337No Hit
CAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGA4661690.13430687767298038No Hit
GAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAG4646930.13388163071008644No Hit
GTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCC4602010.13258745092870453No Hit
CACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA4596590.1324312965561513No Hit
CAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCT4518270.13017483706198762No Hit
ATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCA4440840.12794401915298492No Hit
CCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAG4438040.12786334899742235No Hit
AGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGT4370930.1259298582332073No Hit
ACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC4368820.12586906750883697No Hit
GAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAG4312130.12423578496639394No Hit
CAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCA4303080.12397504749930786No Hit
GAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCA4233420.1219680892719912No Hit
CAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCC4221310.1216191908491832No Hit
CATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCC4168540.12009884652452772No Hit
GCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGG4135560.11914866733508038No Hit
GCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGG4119430.11868394961750044No Hit
AGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAG4063230.11706478435228086No Hit
AGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGG4021270.11585588444963649No Hit
GTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCA4020860.11584407203400053No Hit
CAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCA3999260.1152217594053752No Hit
ACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAG3951070.1138333684066042No Hit
CAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAG3897370.11228623006599402No Hit
AGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAG3862160.11127180285979503No Hit
AAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACA3806760.10967568621045047No Hit
CTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACC3707220.10680786218020212No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGGG3635370.1047378083669276No Hit
AGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG3593850.10354158520301449No Hit
AAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCACGG3536860.10189965942961833No Hit
GTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAA3482890.10034474218115032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)