FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8297649
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49450.046.1223451
GTATCAA130550.044.6908871
ATCAACG188100.031.0287513
TCAACGC192450.030.506824
TATCAAC192800.030.2752632
CAACGCA198400.029.7143465
AACGCAG206350.028.586616
ACGCAGA241050.024.3571287
CGCAGAG248000.023.6467198
AGAGTAC252850.022.93337211
AGTACAT247050.022.60525713
GCAGAGT263050.022.1230589
CAGAGTA265300.021.93543410
AATCCGG22800.021.78601319
GAATCCG22650.021.77799818
GAGTACA259300.021.65757412
CGGATCC26100.019.95611840
AGCGGAT27550.018.53096638
GCGGATC27850.018.2070539
ATCCGGC27100.018.20190420
Produced by FastQC (version 0.11.5)