FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10423143
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154300.1480359618974814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63950.046.308381
GTATCAA176800.045.721691
TATCAAC244750.033.061662
ATCAACG244950.032.906833
TCAACGC247700.032.581864
CAACGCA251850.032.0003625
AACGCAG264650.030.5302376
ACGCAGA311550.025.9341627
CGCAGAG327900.024.6514138
AGAGTAC338700.023.84545711
GAGTACT149400.023.4519612
GCAGAGT355800.022.8056059
CAGAGTA360900.022.47388510
ACGGTCG3850.021.49784349
ACTTTTT168950.021.0244916
AGTACTT168350.020.95562413
GTACTTT170050.020.74652914
TACTTTT171500.020.69177215
AGTACAT223050.020.51666613
CTAGGAC39700.020.416543
Produced by FastQC (version 0.11.5)