FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10423143
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103300.042.3768421
GGTATCA43650.036.78791
TATCAAC147800.029.5023162
ATCAACG149500.029.0952423
TCAACGC150200.029.0052974
CAACGCA154950.028.405815
AACGCAG161000.027.2530776
ACGCAGA196300.022.2468097
TCGGATA7700.021.50287248
CGCAGAG210100.020.7856718
AGAGTAC212950.019.94015311
AGTACAT207400.019.5087313
CCGCTTA8150.019.46883656
CGGATAA8650.019.14119549
GCAGAGT229150.018.8621759
ATAACCG8650.018.742652
CAGAGTA232250.018.5508210
TAACCGC8450.018.36942353
CTAGGAC41450.018.1417853
GTGTACG28850.018.0290091
Produced by FastQC (version 0.11.5)