FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10205187
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA126400.041.162631
GGTATCA50650.039.636831
ATCAACG174150.029.5302623
TCAACGC175950.029.2471924
CAACGCA178650.028.8007935
TATCAAC185150.028.0748582
AACGCAG191500.026.8854246
TCTAGCG10400.023.54413828
CTAGCGG10950.022.67906429
TAACCGC7150.022.18658353
ACGCAGA232300.022.1781397
TACCGGT12900.021.9203727
CCGCTTA7050.021.52349756
AATCCGG20050.021.32716819
ATACACT28450.021.2158474
GTACTAG7600.021.2064481
AATACGA11300.021.07124939
CGCAGAG245850.021.0255178
CGCTTAC7250.020.92943857
ACGAAAG23200.020.66217856
Produced by FastQC (version 0.11.5)