FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10205187
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99500.041.5381931
GGTATCA42250.032.166871
ATCAACG132650.030.6074753
TCAACGC137650.029.8456524
CAACGCA140800.029.2520085
TATCAAC147750.027.877122
AACGCAG150600.027.5549076
TCTAGCG9900.022.99610128
TCGGATA7250.022.3611848
ACGCAGA183900.022.359027
CTAGCGG10800.021.7185429
CGCAGAG193550.021.226538
TAACCGC8150.020.73790453
CCGCTTA7700.020.6060956
AGTACAT197000.020.3108813
GAATCCG21450.020.262318
AGAGTAC204300.020.00771911
CGCTTAC8250.019.65025157
AAAGTCG22650.019.64481259
GAACGAA23200.019.6251254
Produced by FastQC (version 0.11.5)