FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10554333
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93350.043.716471
GGTATCA38950.035.7610551
ATCAACG138650.029.4813773
CAACGCA143600.028.9697135
TCAACGC142950.028.642284
TATCAAC144650.028.3784142
AACGCAG154650.026.7882566
ACGAAAG21300.021.53838356
TACCGGT14600.021.5002757
ATTAATC21800.021.3617545
ACGCAGA196250.021.0221427
AATCCGG24650.020.8502119
CTAGGAC42350.019.9554713
GAATCCG25700.019.72963518
CGCAGAG209050.019.6526538
TATCGTC21550.019.36774863
GAACGAA23350.019.2044454
AGTACAT205550.019.14692113
CTAGTAC17900.019.0779383
AAAGTCG25000.019.04075859
Produced by FastQC (version 0.11.5)