FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10626186
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58600.045.0004841
GTATCAA155550.044.9455031
ATCAACG228350.030.4965483
TATCAAC230750.030.4337522
TCAACGC233850.029.7787274
CAACGCA239250.029.1460135
AACGCAG251350.027.756256
ACGAAAG20850.022.9920256
ACGCAGA304200.022.956717
CGCAATA9800.022.18172836
AAAGTCG22250.022.01085559
GAGTACT130650.021.9879912
CGCAGAG318950.021.9165868
AGGTTCG22300.021.80612667
GAACGAA22400.021.70898254
TCTAGCG10050.021.6198728
AGAGTAC324000.021.50088511
TACCGGT13100.021.3234547
AATCCGG27900.021.13712719
CTAGCGG10550.020.92322229
Produced by FastQC (version 0.11.5)