FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10626186
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99650.041.829131
GGTATCA44350.036.8386761
ATCAACG144050.028.8549333
TCAACGC148850.028.1560484
TATCAAC152750.027.4613022
CAACGCA153500.027.4158575
AACGCAG165250.025.6335856
TCTAGCG11600.024.3830428
AATACGA12300.023.55669439
CGCAATA12500.022.35214836
CTAGCGG12700.022.27112229
AATCCGG29050.021.61083419
GAATCCG29550.021.12843718
ACGCAGA202300.020.7514867
CGCAGAG214200.019.5503128
CTAGGAC45600.019.5164643
AAAGTCG23500.019.37538359
AGAGTAC220150.018.9904111
TACCGGT14900.018.9837517
ATACGAA14900.018.98312840
Produced by FastQC (version 0.11.5)