FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11266670
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA169600.15053249984245567No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG156740.1391183020360053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA143850.045.241141
GGTATCA56850.041.7339741
ATCAACG216500.030.0473023
TCAACGC219900.029.6291224
CAACGCA225050.028.962955
TATCAAC228200.028.5832122
AACGCAG241400.027.014266
CGCAATA16200.026.8374436
TCTAGCG16550.026.2562428
CTAGCGG17250.025.1921129
AATACGA18000.024.34528439
AATCCGG22450.023.5032219
ATTAATC33600.022.79226145
TATATCG19400.022.22163260
ATAACCG6850.022.15207152
CAATACG19900.022.02095838
ACGCAGA298700.021.8436227
ACGAAAG33850.021.80284556
GAACGAA34400.021.45453854
AGGTTCG36700.021.3314267
Produced by FastQC (version 0.11.5)