FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10101929
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA137000.044.5422441
GGTATCA53250.044.4046751
ATCAACG200900.030.285253
TATCAAC203750.030.0484332
TCAACGC205800.029.6301824
CAACGCA208850.029.144325
AACGCAG220400.027.6479036
AAAGTCG22200.025.47733159
ACGAAAG22300.025.20780456
GAACGAA22350.024.68862354
AGGTTCG23850.024.00347567
ATTAATC24100.023.76059345
CTAGCGG9350.023.60847529
CTAGGAC40000.023.3680133
CGGTCGA6150.023.00346620
AGAACGA24200.022.65842653
TCTAGCG9450.022.62667728
ACGCAGA269700.022.5938747
GAATCCG21900.021.7308418
AGTCGGA25900.021.70455461
Produced by FastQC (version 0.11.5)