FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10101929
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84450.041.9113431
GGTATCA35750.036.1615831
ATCAACG123800.029.0885183
TCAACGC127100.028.3050084
CAACGCA132000.027.7518755
TATCAAC129150.027.644392
AACGCAG140150.025.990766
AAAGTCG24100.021.8978359
CTAGGAC41700.021.5896783
ATTAATC25950.021.53490845
AATACGA12500.020.97205539
CGCAATA12200.020.92260636
TATATCG19050.020.64117460
AGGTTCG25150.020.57216367
GAACGAA25800.020.45494854
ACGAAAG25700.020.40032856
AGTCGGA27050.020.274861
ACGCAGA178300.020.178147
CGGTTCG30850.020.01406318
TCTAGCG12800.019.94156328
Produced by FastQC (version 0.11.5)