FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10751574
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA112200.042.4432261
GGTATCA46300.038.8056341
ATCAACG159100.030.3747523
TCAACGC164850.029.4826724
CAACGCA167500.028.9548535
TATCAAC166700.028.8059862
AACGCAG181000.026.7953626
ACGCAGA221050.021.8625357
AAAGTCG23400.021.5219659
CGCAGAG235200.020.5912488
ACGAAAG24400.020.49835256
CGGTCGA9850.020.31143620
AGGTTCG26150.019.78610667
AGAGTAC240800.019.71100411
AGTCGGA27150.019.69276461
AATCCGG24050.019.6497219
GAACGAA26150.019.25874554
GCAGAGT251100.019.1501859
GAATCCG25050.019.14061518
CAGAGTA249950.019.07259610
Produced by FastQC (version 0.11.5)