FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11079485
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95750.041.1948361
GGTATCA42950.037.0114251
ATCAACG137500.028.5727443
TCAACGC138850.028.3451354
CAACGCA140250.027.9886575
TATCAAC142450.027.4858652
AACGCAG150750.026.1308426
ACGCAGA188450.020.5374857
CGCGAAT5600.019.7105528
CGCAATA10100.019.46722236
CGCAGAG201050.019.2332218
CGGTTCG29150.019.16926418
CTAGGAC38000.018.6080633
TTCGCTA29900.018.57323521
AGAGTAC206750.018.55247911
TACCGGT17350.018.2910617
GAATCCG27600.018.24621418
GGTTCGC31150.018.16011619
AGTACAT202650.018.07659713
TATCGTC20000.017.93627563
Produced by FastQC (version 0.11.5)