FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11086271
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA150840.13606017749340604No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG143940.12983626324848094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122000.044.369991
GGTATCA49200.042.8421361
ATCAACG180350.029.7760413
TCAACGC184800.029.0397154
CAACGCA187550.028.5560385
ATTAATC32600.027.6183545
TATCAAC195000.027.5924572
ACGAAAG33250.026.65659356
AAAGTCG33750.026.56883659
AACGCAG203150.026.4136526
GAACGAA34200.026.11769554
AGGTTCG36550.024.53258167
AGTCGGA37800.023.813261
AGAACGA39700.022.5853353
CTAGGAC48350.022.1849463
AACGAAA40900.022.00788955
TCTAGCG13350.021.69958328
TCGGATA7350.021.58466748
TACCGGT14450.021.4790127
TAACCGC7250.021.4051453
Produced by FastQC (version 0.11.5)