FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11086271
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA137770.12427082109033778No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG126870.1144388406164706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103950.042.523251
GGTATCA46450.037.4631271
CGCAATA14400.028.9851836
ATCAACG156150.028.2536013
TCAACGC160950.027.4959774
TATCAAC164050.027.0633072
CAACGCA164300.027.0622865
ATTAATC30250.026.79745945
AAAGTCG31400.026.14417359
AATACGA16050.026.00480739
TCTAGCG16100.025.92381328
ACGAAAG31550.025.36402756
CTAGCGG16800.025.25417929
AACGCAG178150.024.8228346
GAACGAA32550.024.47893554
AGGTTCG33650.023.4729867
CAATACG18250.023.0595138
AGTCGGA35500.022.73595261
AGAACGA35500.022.63899653
ATACGAA18850.021.40976540
Produced by FastQC (version 0.11.5)