FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9928233
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA118620.11947745384299502No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG110950.11175201065486678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA143700.045.994011
GGTATCA55350.042.6057621
ATCAACG209250.031.6667293
TCAACGC215000.030.802854
CAACGCA218800.030.2470915
TATCAAC220200.030.2339152
AACGCAG231350.028.5760386
ACGCAGA276950.023.8708537
CGCGAAT4350.023.78440728
CGCAGAG292000.022.6636898
AGAGTAC300300.022.03750811
ACGAAAG30950.021.61729856
TCTAGCG14750.021.51077828
AGGTTCG31150.021.4781867
CAGAGTA310450.021.40576210
ATTAATC31600.021.17861245
CTAGCGG15450.020.9843129
GAGTACT77900.020.98378212
GCAGAGT318250.020.9244739
AAAGTCG32250.020.85326259
Produced by FastQC (version 0.11.5)