FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11355718
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173620.15289213768781507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA198500.044.7496571
GGTATCA73850.044.6989751
ATCAACG269800.032.714823
TATCAAC272900.032.496012
TCAACGC271550.032.4778754
CAACGCA276900.031.8612775
AACGCAG290450.030.3981086
ACGCAGA345250.025.6131087
CGCAGAG363600.024.3109938
GAGTACT171800.024.09006312
AGAGTAC371150.023.73282211
CAGAGTA388600.022.78248210
GCAGAGT389600.022.715159
GTACTTT189000.021.97073414
AGTACTT195000.021.4538913
ACTTTTT196650.021.44954516
TACTTTT198250.021.0850715
ACGAAAG22200.019.88656656
AATCCGG20800.019.73226219
AAAGTCG22450.019.5116559
Produced by FastQC (version 0.11.5)