FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11355718
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115150.040.5163961
GGTATCA49800.038.0744061
ATCAACG159200.029.317293
TCAACGC162750.028.804984
CAACGCA166650.028.3588225
TATCAAC164650.028.1801342
AACGCAG174400.026.8415896
ACGCAGA211000.022.0714057
CGCGAAT5700.021.18158528
CGCAGAG226000.020.606498
AGAGTAC230000.020.06804511
AGTACAT234250.019.05548313
CAGAGTA243400.019.0483510
GCAGAGT245800.018.792279
AAAGTCG21750.018.71475659
AATCCGG22300.018.40811319
GAGTACA249600.017.98050112
ATACCGT21500.017.6493646
ACGAAAG23200.017.54508656
AGTCGGA25600.017.24763561
Produced by FastQC (version 0.11.5)