FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10796921
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG147900.1369834974248677No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA145050.13434385599375973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66800.046.754921
GTATCAA175250.045.1625941
ATCAACG253400.031.1988073
TCAACGC257150.030.7967784
CAACGCA262150.030.190365
TATCAAC266850.029.769592
AACGCAG274100.028.974396
AAAGTCG34850.026.42348959
AGGTTCG35650.025.82814467
ACGAAAG35450.025.68392256
AGTCGGA37600.024.76587161
GAACGAA37150.024.69348554
ATTAATC38500.024.10337645
AGAACGA38300.024.04163653
ACGCAGA334550.023.770037
TCTAGCG15100.023.2959728
AAGTCGG40400.023.22026860
CGCAGAG347450.022.8973228
TACCGGT10850.022.5662847
AGAGTAC359200.022.11992311
Produced by FastQC (version 0.11.5)