FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10796921
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA124000.11484755700259361No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG123940.11479198560404398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54850.042.2509731
GTATCAA127500.041.662921
ATCAACG183150.029.362033
TCAACGC188500.028.693244
CAACGCA193200.027.9241855
AACGCAG200950.026.9161536
TATCAAC205750.026.2392582
TCTAGCG18250.024.38258628
CTAGCGG18850.023.60626229
AAAGTCG31750.023.2490359
CGCAATA18850.022.87449636
AATACGA19800.021.95048739
ATTAATC34300.021.82360845
ACGCAGA247500.021.7144687
AGGTTCG33600.021.55821667
ACGAAAG33450.021.5521756
GAACGAA34100.021.44501354
AGTCGGA34600.021.23431861
CGCAGAG257450.020.8351428
AGAACGA36000.020.12156153
Produced by FastQC (version 0.11.5)