FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11008241
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG113350.10296831255783734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63450.044.8404161
GTATCAA154150.042.7027661
ATCAACG214050.030.6488423
TATCAAC217000.030.360592
TCAACGC219700.029.8602434
CAACGCA224300.029.2121965
AACGCAG242950.027.0119446
TACCGGT16400.023.9714877
CGCAATA10250.023.5651736
ACGCAGA297550.022.078477
AAAGTCG20900.021.12171759
CGCAGAG313250.020.927688
TCTAGCG11550.020.90179428
CTAGGAC52150.020.9002533
CTAGCGG11600.020.81368429
AGAGTAC322550.020.32464611
TATATCG18750.020.23284560
ACGAAAG21900.020.1569856
CAGAGTA330250.019.89226210
AATACGA12200.019.79914539
Produced by FastQC (version 0.11.5)