FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11008241
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA112350.041.4650731
GGTATCA48100.037.390551
ATCAACG163700.028.5732293
TCAACGC166000.028.1560464
TATCAAC166400.028.0489392
CAACGCA169350.027.9048545
AACGCAG183800.025.7116136
ACGCAGA223800.021.07017
TCTAGCG10150.020.05119328
CGCAGAG234800.019.9360898
CGCAATA10400.019.9002436
AGAGTAC242300.019.1619611
AATACGA11100.018.95602639
AGTACAT242150.018.70323613
CAGAGTA250450.018.66261710
ATACCGT20200.018.6135586
CTAGGAC53100.018.5788923
CTAGCGG11450.018.07590329
GCAGAGT260950.017.9514989
TACCGGT16200.017.8863817
Produced by FastQC (version 0.11.5)