FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10854279
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG165450.15242836488724862No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA132320.12190584008389686No Hit
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG126660.11669130671876041No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG119280.10989214483983689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71300.047.36431
GTATCAA197000.045.589141
ATCAACG289800.031.3762873
TATCAAC290400.031.3365152
TCAACGC295300.030.7441844
CAACGCA302400.030.205275
AACGCAG320750.028.4557296
ACGCAGA402650.022.6334977
CGCAGAG417400.021.775838
AGAGTAC422350.021.4144411
TATCGTC21700.021.29990263
CAGAGTA428950.021.1493810
AGTACAT424000.021.11880713
ATACCGT39900.020.9213166
CGCAATA14350.020.91249736
GCAGAGT438800.020.6196949
CGTCGTA35600.020.44471410
ACCGTCG40400.020.4062428
GAGTACA446400.020.06736212
CGAAATT12300.019.91066648
Produced by FastQC (version 0.11.5)