FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10050194
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120630.12002753379686004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61700.046.4871141
GTATCAA173550.045.7313161
TATCAAC246250.032.5099072
ATCAACG252050.031.6652323
TCAACGC255550.031.2711034
CAACGCA262400.030.4777165
AACGCAG278550.028.7223226
ATAACCG6600.024.55897552
ACGCAGA332400.024.1005657
TCGGATA6600.024.03848
CGCTTAC7050.023.48067557
CACGCGA5600.023.40112513
CGCAGAG343850.023.2778558
TAACCGC7250.022.83281753
CCGCTTA7500.022.53177656
AGAGTAC357150.022.40190911
CGCGAAT9050.021.7222228
GAGTACT145250.021.5581212
GCAGAGT371600.021.5580949
CAGAGTA374550.021.50810210
Produced by FastQC (version 0.11.5)