FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10050194
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111100.044.2226261
GGTATCA47300.036.6171881
ATCAACG160000.030.7212943
TATCAAC159850.030.6445142
TCAACGC166400.029.726574
CAACGCA169150.029.1621465
AACGCAG181100.027.1239816
ACGCAGA219400.022.2319157
CGCAGAG231400.020.9746538
AGAGTAC240200.020.11935611
AGTACAT239550.019.61206813
CTAGGAC44700.019.3691623
CAGAGTA253350.019.29310810
GCAGAGT251950.019.2091169
GAGTACA257500.018.28530512
AATCCGG20250.017.2043719
CGCAATA8300.017.03924636
CTCTAGG51350.016.660581
CGGTTCG20300.016.48223118
TTCGCTA20300.016.48223121
Produced by FastQC (version 0.11.5)