FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12353915
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA168110.13607832011147883No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG154460.1250291911511452No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA128600.10409655562629337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA155550.045.9688951
GGTATCA58450.041.9552841
ATCAACG225550.031.6406063
TCAACGC229900.030.995914
CAACGCA233450.030.4910685
TATCAAC245250.029.2965262
AACGCAG248200.028.7198036
TATCGTC24000.023.85329663
ATTAATC40850.023.39106645
ACGCAGA310750.022.9056747
ACGAAAG41450.022.88139756
TCTAGCG17600.022.14256128
CTAGCGG18000.022.03592929
GAACGAA43050.021.9504354
CGCAGAG327650.021.7449998
AATCCGG32150.021.66780319
AAAGTCG44900.021.35368559
CGCAATA17950.021.14571636
GAATCCG33400.020.96013518
AGAGTAC341100.020.88798911
Produced by FastQC (version 0.11.5)