FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12353915
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA148310.12005101216901687No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG137900.11162453359926792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA136600.043.7407761
GGTATCA55400.040.53111
ATCAACG203800.029.619873
TCAACGC211600.028.7072244
CAACGCA215800.028.052725
TATCAAC225500.026.8785692
AACGCAG231000.026.1919986
CGCAATA17900.023.31757436
ATTAATC41350.023.0244845
AAAGTCG42850.022.13682459
TCTAGCG19900.021.66710528
ACGCAGA277550.021.6127197
ACGAAAG43650.021.33634456
AGTCGGA45550.021.20302461
AATACGA19750.021.13279739
GAACGAA44250.020.89138654
CTAGCGG20650.020.7130429
AGGTTCG45300.020.63475267
CGAAATT15550.020.63029148
CGCAGAG293650.020.4982348
Produced by FastQC (version 0.11.5)