FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10410466
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89700.041.6719861
GGTATCA39100.036.54261
ATCAACG129250.028.4493943
TCAACGC135200.027.3246734
CAACGCA136350.027.2719525
TATCAAC137900.026.7662012
AACGCAG145600.025.3497056
ATTAATC23750.023.67461245
AGGTTCG24400.022.900867
ACGAAAG23950.022.89958256
AAAGTCG24450.022.854359
GAACGAA25250.022.13083854
CTAGGAC36950.021.751433
GAATCCG22950.021.19232418
AGTCGGA27400.020.7713761
CGCGAAT5000.020.69603728
CCGCTTA6950.020.3486256
ACGCAGA184650.019.8954127
AGAACGA28700.019.3501453
TATCGTC20850.019.19029263
Produced by FastQC (version 0.11.5)