FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10749326
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA124660.11597006175084838No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG113370.10546707765677589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122850.045.105221
GGTATCA48600.038.197151
ATCAACG186350.029.8746813
TCAACGC189450.029.4579814
CAACGCA195050.028.648795
TATCAAC194900.028.5662442
AACGCAG207350.026.799636
TATATCG21800.023.89095160
CGCAATA14400.022.75633836
GTACTAG7450.022.0553681
TATCGTC17250.021.39497263
GAATCCG29400.021.11896118
TACCGGT25300.020.996147
ACGCAGA265400.020.8079687
CTAGGAC45900.020.7408033
ATTAATC30300.020.60579345
AATACGA15950.020.11191639
TTATATC26750.019.98623759
CGCAGAG276700.019.9208078
AATCCGG30950.019.83840419
Produced by FastQC (version 0.11.5)