FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10502868
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108650.10344793441181971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56950.045.1094741
GTATCAA153350.044.7341
TATCAAC217150.031.7526152
ATCAACG217250.031.6099323
TCAACGC219800.031.242024
CAACGCA223050.030.7984495
AACGCAG234050.029.276886
ACGCAGA284800.024.071997
CGCAGAG300700.022.7992528
CACGCGA5550.022.3690313
GAGTACT129500.022.10273212
AGAGTAC311200.022.05236811
CAGAGTA323500.021.26699810
GCAGAGT328500.020.95389
TCGGATA5550.020.50738548
GAATCCG27600.020.36707118
ACTTTTT144400.020.22842616
TACTTTT144200.020.11270115
AATCCGG27850.020.06031619
GGTCTAG23650.019.8307481
Produced by FastQC (version 0.11.5)