FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10017138
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108850.041.6101071
GGTATCA48200.038.5435681
ATCAACG155950.029.5946883
TCAACGC159100.029.0519644
CAACGCA162050.028.6933825
TATCAAC165650.027.8853152
AACGCAG170400.027.044836
ACGCAGA207350.022.1423457
TATCGTC17450.021.3482763
CGCAGAG217850.021.1226228
TACCGGT9300.020.399967
AGAGTAC224850.020.29587711
CGCAATA12700.020.09874336
AATACGA12600.019.98439839
CTAGGAC33050.019.8301123
AGTACAT219950.019.80657813
TCTAGCG12400.019.47268928
GCAGAGT240500.019.1189759
CAGAGTA243350.018.80992110
AGGTTCG22450.018.7443967
Produced by FastQC (version 0.11.5)