FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10433501
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96550.041.8310471
GGTATCA42800.034.9074131
ATCAACG138750.029.2610173
TCAACGC142650.028.5096654
CAACGCA144600.028.292325
TATCAAC152000.026.689912
AACGCAG154250.026.4106456
ATTAATC28700.022.11516645
AAAGTCG28700.021.8738459
AGGTTCG28900.021.24504967
CGCAATA11750.021.13696736
ACGCAGA194250.020.9544017
TCTAGCG11900.020.87073528
ACGAAAG29550.020.54446856
GAACGAA30450.020.05061754
CGCAGAG204650.019.8053478
AGTCGGA32850.019.4255861
AGAGTAC209500.019.26387211
GAATCCG23650.019.25220318
AGTACAT203150.019.18656213
Produced by FastQC (version 0.11.5)