FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11336150
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA206450.1821165033984201No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG196060.1729511342034112No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA163070.14384954327527424No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCG114890.10134834136810117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109350.042.2751581
GGTATCA48600.035.9736141
TCTAGCG21950.028.28862628
ATTAATC49650.028.0002345
AAAGTCG49450.027.90357859
CGCAATA21800.027.85043536
ATCAACG168150.027.4702953
ACGAAAG49700.027.34665356
AGGTTCG49100.027.11842267
TCAACGC172150.026.8724424
AATACGA23050.026.78873439
GAACGAA50650.026.766154
CAACGCA173650.026.5208855
CTAGCGG23450.026.4791229
AGTCGGA53500.025.9202161
AGAACGA54500.024.93857853
CAATACG24600.024.82069838
AACGCAG190300.024.3998876
TATCAAC190400.024.3701362
AACGAAA56200.024.1842155
Produced by FastQC (version 0.11.5)