FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9618337
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94950.041.8112031
GGTATCA43500.036.3460921
ATCAACG131900.030.2605953
TCAACGC135650.029.5001894
CAACGCA138100.029.0767575
TATCAAC140100.028.5883942
AACGCAG144550.027.7793146
ACGCAGA177150.022.608817
AATCCGG21450.022.03320519
CGCAGAG184850.021.5737178
GAATCCG22950.020.7434418
AGTACAT190850.020.29876913
AGAGTAC200800.019.65380911
GCAGAGT211000.018.8510599
CGCAATA8650.018.74426536
CAGAGTA217000.018.28213910
GAGTACA214500.018.1250312
TCTAGCG9100.017.81781228
TGCGCTA17100.017.55168525
AAAGTCG19700.017.51156459
Produced by FastQC (version 0.11.5)