FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10470704
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA177450.16947284537887805No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG174940.16707568087112387No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA126170.12049810595352518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113750.045.130291
GGTATCA45050.036.8214651
ATCAACG174600.029.3180393
TCAACGC179800.028.4309464
CAACGCA183600.027.9940095
TATCAAC191100.026.8067532
AAAGTCG39050.026.41126659
CTAGCGG18800.026.05370729
ACGAAAG40200.025.91350656
TCTAGCG19100.025.82532928
AACGCAG198150.025.799046
AGTCGGA40650.025.37146461
AGGTTCG40400.025.27196567
GAACGAA42050.024.93737854
CGCAATA19600.024.81513236
ATTAATC42700.024.71968745
AGAACGA44150.023.673153
AATACGA20600.023.27483639
AAGTCGG45750.022.61856760
ACCGTCG30150.021.9668338
Produced by FastQC (version 0.11.5)