FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10740760
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA174320.1622976400180248No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG169040.15738178676369272No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA128690.11981461274621164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65800.048.1342961
GTATCAA170650.044.2154851
ATCAACG246750.030.5702783
TCAACGC252000.029.9470834
CAACGCA254550.029.6434945
TATCAAC261700.029.034782
AACGCAG269200.028.0683216
AAAGTCG37650.025.64852559
ACGAAAG37150.025.25104956
ATTAATC38850.024.50656345
AGGTTCG38900.024.29182867
GAACGAA38600.024.21281454
ACGCAGA329350.022.9735477
AGAACGA41250.022.8244353
GTACTAG5450.022.4751951
AATCCGG22000.022.41695619
GAATCCG21950.022.31100518
CTAGGAC45300.022.3088473
AGTCGGA42550.022.28929961
CGCAGAG344800.021.934038
Produced by FastQC (version 0.11.5)