FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10740760
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA150170.13981319757633537No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG148730.1384725103251539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA129550.043.0431251
GGTATCA52600.042.8164061
ATCAACG183900.030.5550233
TCAACGC189100.029.9336934
CAACGCA193800.029.2433435
AACGCAG204700.027.551246
TATCAAC204850.027.4494292
AAAGTCG36200.024.67927659
ATTAATC38500.023.83264545
ACGAAAG38650.023.38250256
AGGTTCG39500.023.05366367
AGTCGGA38950.023.02518361
GAACGAA39600.022.73445354
ACGCAGA248500.022.6397087
CTAGCGG15850.022.41564429
AGAACGA40400.022.36975353
CGTCGTA28100.022.21874610
TCTAGCG15850.021.98059528
ACCGTCG30800.021.838828
ATACCGT33500.021.7259546
Produced by FastQC (version 0.11.5)