FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10490601
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA130050.039.914331
GGTATCA53300.039.336681
ATCAACG171950.029.7870853
TCAACGC175150.029.2615914
CAACGCA177450.028.8794295
TATCAAC187450.027.5462762
AACGCAG190200.027.0336466
AAAGTCG20750.024.93147359
TCTAGCG9100.024.25463328
ACGAAAG22100.022.4716356
CTAGCGG10200.022.31664529
ACGCAGA231550.022.2952547
GAACGAA23200.021.7032754
CGCAGAG243800.021.1608588
TAACCGC6050.021.09125753
AGGTTCG24150.020.99152267
TACCGGT14500.020.6912487
TCGGATA6050.020.52249548
AGAGTAC252550.020.4005911
CTAGGAC36250.020.3614883
Produced by FastQC (version 0.11.5)