FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12672799
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA323130.25497918810201287No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG302130.23840826324160905No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA202310.15964113373848982No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCG194550.1535177824567406No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG194090.15315480029313178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150660.11888454949849675No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA144280.11385014470757407No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCG142620.11254025255194215No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCG138770.10950224966086812No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGG133620.10543842761176912No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGT129620.10228206097169219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98850.051.542911
GTATCAA290050.046.3070831
ATCAACG421350.032.08163
TCAACGC431450.031.3539524
CAACGCA439300.030.7528425
TATCAAC444000.030.546722
AACGCAG462800.029.235886
AAAGTCG64500.025.61480359
ACGAAAG64250.025.33928556
AGGTTCG66100.024.52485567
AGTCGGA67000.024.50458561
GAACGAA66500.024.4819454
ACGCAGA553900.024.4336787
AGAACGA68450.024.13695153
CGCAGAG567700.023.8640298
ACCGTCG53900.023.6757728
ATTAATC70400.023.66744245
ATACCGT57450.023.5335396
AGAGTAC574450.023.51785511
CGTCGTA53500.023.27267610
Produced by FastQC (version 0.11.5)