FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10918617
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA128800.11796365785153924No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG122730.11240434571521284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80900.042.4006651
GGTATCA35450.037.0030631
ATTAATC27650.030.93963845
ACGAAAG27300.029.81333456
CGCAATA12550.028.04249236
ATCAACG122700.027.8296363
GAACGAA29750.027.47391754
CAACGCA125950.027.2434585
TCAACGC125500.027.2077394
AAAGTCG30850.026.94207459
TCTAGCG13600.026.37155528
AGGTTCG31450.026.31805467
AATACGA13250.026.30228439
TATCAAC134250.025.6929822
AACGCAG136250.025.1584136
TATATCG15350.024.93915660
TACCGGT16750.024.7058497
AGAACGA33400.024.5746753
CTAGCGG14900.024.07299629
CATTAAT36700.023.40399444
Produced by FastQC (version 0.11.5)