FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10944237
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161800.1478403656645959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69550.044.877291
GTATCAA178550.044.7495461
TATCAAC247800.032.3788342
ATCAACG251100.031.8697133
TCAACGC254950.031.4281774
CAACGCA258850.030.963885
AACGCAG272300.029.4716386
ACGCAGA328800.024.41777
CGCAGAG344350.023.3148428
AATCCGG21550.023.04478819
GAGTACT162500.022.941412
AGAGTAC357200.022.41828311
GAATCCG22350.021.91131218
GCAGAGT369350.021.811349
CTAGGAC47350.021.5629733
CAGAGTA374450.021.4591110
ACTTTTT181850.020.84221616
CGCAATA7300.020.79809436
GTACTTT181400.020.66540114
AGTACTT182000.020.63507713
Produced by FastQC (version 0.11.5)