FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10944237
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105950.041.019621
GGTATCA46600.037.275031
ATCAACG147850.029.8400273
TCAACGC149650.029.4588664
TATCAAC149500.029.3962612
CAACGCA154200.028.791355
AACGCAG164000.027.0286986
ACGCAGA200500.021.9879287
CTAGGAC48300.020.4253223
CGCAGAG217200.020.3609498
TACCGGT13400.020.079727
AGAGTAC222350.019.59368911
AGTACAT220250.019.15343313
AATCCGG23650.018.9610419
GCAGAGT234900.018.635739
GAATCCG24700.018.57388318
CTCTAGG53500.018.3200681
CAGAGTA240400.018.20920810
TATATCG14450.017.90339960
GAGTACA241550.017.69319712
Produced by FastQC (version 0.11.5)