FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9993277
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA138500.045.3714561
GGTATCA53050.043.817821
ATCAACG194000.032.430523
TATCAAC195200.032.3381462
TCAACGC195500.032.2339744
CAACGCA199100.031.6292295
AACGCAG210350.029.9206316
ACGCAGA253950.024.7973427
CGCAGAG268250.023.4236558
AGAGTAC273900.022.91540711
CAGAGTA288150.021.80609710
GCAGAGT299350.021.128489
AAAGTCG16350.021.09356559
ACGAAAG16350.021.09314556
GAGTACT115200.020.6257912
CGCGAAT6900.020.49285928
CGCAATA9500.019.97672336
AGTACAT198800.019.6022513
CTAGGAC40150.019.5016383
TCTAGCG10200.018.93457828
Produced by FastQC (version 0.11.5)