FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10486473
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA134810.1285560931687899No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG134550.12830815470559073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA150350.043.4649121
GGTATCA60650.041.0910381
ATCAACG213900.030.4590243
TCAACGC216100.030.1798464
CAACGCA220500.029.5897335
TATCAAC225200.029.1003762
AACGCAG234650.027.819966
TCTAGCG14500.024.49766228
CGCAATA14650.023.78997436
CTAGCGG15150.023.67672729
AATACGA15400.022.8571939
ACGCAGA285900.022.8090887
AAAGTCG32150.022.20490559
CGCGAAT5500.021.94627428
CGCAGAG297050.021.941438
AGGTTCG32950.021.87459867
ACGAAAG32800.021.55509656
AGAGTAC303250.021.48176811
GAACGAA32900.021.38444554
ATTAATC34050.021.1730845
Produced by FastQC (version 0.11.5)