FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10486473
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA113910.10862565516546888No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG111870.10668029183882893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110250.042.1144831
GGTATCA47650.035.460181
ATCAACG158500.029.5336593
TCAACGC162300.028.9484444
CAACGCA164750.028.5807695
TATCAAC167400.027.7795962
AACGCAG175400.026.7475666
GAACGAA33650.023.78134554
ACGAAAG33850.023.02932556
ATTAATC34050.022.69210445
AAAGTCG33550.022.61881859
AGGTTCG33250.022.51103267
TCTAGCG15250.022.39305728
CTAGCGG15500.022.25442329
ACGCAGA216700.021.6021987
AGAACGA37400.021.30452353
CGCAATA15800.021.1766136
AGTCGGA36000.020.8877561
CGCAGAG231050.020.2903968
AGAGTAC231500.019.9818711
Produced by FastQC (version 0.11.5)