FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10620255
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG122690.1155245330738292No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA122490.11533621367848512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA119000.041.4604761
GGTATCA49100.038.1173251
ATCAACG168200.029.243983
TCAACGC169500.029.079514
CAACGCA171600.028.639065
TATCAAC179750.027.6729642
AACGCAG184700.026.6451536
AAAGTCG26850.024.92065659
CTAGGAC39150.024.2294673
ACGAAAG27950.024.18611156
GAACGAA29250.022.87502354
ATTAATC29450.022.7244945
AATTACG5850.022.4036956
TACCGGT19250.022.3944537
TCGGATA6500.022.28619648
AGTCGGA30200.022.27038261
TCTAGCG11550.022.09741828
AATCCGG25550.022.00198719
AGGTTCG30550.021.78831367
AGAACGA30800.021.61166253
Produced by FastQC (version 0.11.5)