FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10751947
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA118270.1099986820991584No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG116780.10861288657765891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102250.040.7445341
GGTATCA48900.036.6353841
TCAACGC147150.028.4800784
ATCAACG146300.028.4574643
CAACGCA150600.028.1034015
AACGCAG160900.026.2402956
TATCAAC163650.025.484252
AAAGTCG33900.024.72589159
AGGTTCG33250.024.06742567
GAACGAA35450.024.03400254
ACGAAAG34950.023.88435756
ATTAATC36700.023.31015445
TCTAGCG17000.023.1312628
AGAACGA38050.022.57293753
AGTCGGA37300.022.56411661
CTAGCGG18450.022.0610929
AAGTCGG38900.021.99071760
ACGCAGA205550.020.3893897
AATACGA19850.020.15769439
ATACCGT28150.019.9734276
Produced by FastQC (version 0.11.5)