FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10085643
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG112240.1112869055547574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA136300.045.139731
GGTATCA53200.043.327411
ATCAACG194300.031.6867793
TATCAAC195300.031.613782
TCAACGC197100.031.2538284
CAACGCA201100.030.5934685
AACGCAG212300.029.0116946
ACGCAGA264950.023.2592547
CGCAGAG276200.022.3116518
AGAGTAC282900.021.74688711
TATCGTC20550.021.31298663
CAGAGTA293900.021.0500810
TACCGGT20100.020.931437
GCAGAGT301400.020.5377129
TATATCG19350.020.49712660
CTAGGAC48100.019.9348243
GAGTACT86600.019.87091412
AAAGTCG17800.019.76315559
AGTACAT225150.019.7278213
CACGCGA6650.019.70593813
Produced by FastQC (version 0.11.5)