FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10085643
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGACAATGGTCTGGACACTTCACTGGGTG101660.10079674642459584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA106600.043.138291
GGTATCA45750.038.5420341
TATCAAC150650.030.4770072
ATCAACG151900.030.2932833
TCAACGC155850.029.7236734
CAACGCA160300.028.8778695
AACGCAG168800.027.3419726
ACGCAGA212450.021.5943977
CGCAGAG223650.020.4667178
TATCGTC19050.020.46082763
CTAGGAC48950.020.2244873
AGAGTAC227900.019.8578111
TACCGGT20700.019.4967257
AGTACAT227550.019.38753713
TATATCG19150.019.27314460
CAGAGTA238750.019.02789310
AAAGTCG17800.018.99100559
ACGAAAG18450.018.69595556
GCAGAGT245350.018.6286039
GAGTACA248450.017.75706912
Produced by FastQC (version 0.11.5)